Total Starch by Perchloric Acid Method

Name of Parameter: Total Starch by perchloric acid method
Working Principle: Starch content can be estimated by first hydrolyzing starch into glucose molecules by treating with dilute acids. Resulting glucose molecules can then be spectrophotometrically estimated by anthrone method. Starch content is calculated by multiplying the thus estimated glucose content by a factor of 0.9. The test sample is first treated with hot 80% ethanol to remove sugars and then starch is extracted with 52% perchloric acid. In hot acidic medium, starch is hydrolyzed to glucose and then dehydrated to hydroxymethyl furfural compound that reacts with anthrone to give green coloured product having the absorption maximum at 630 nm.
Application/Scope: Starch is the storage form of carbohydrate in plants abundantly found in in plant roots, tubers stems and fruits. Starch, which is composed of several glucose molecules, is a mixture of two types of components namely amylose and amylopectin. Starch is hydrolysed into simple sugars by dilute acids and the quantity of simple sugars is measured colorimetrically. Starch content in the sample will beprovided in mg/g or percentage
Sample Requirement: 5gm of brown/white rice
Charges: Rs.1490 per sample