Reducing Sugar by Nelson-Somogyi Method

Name of Parameter: Reducing Sugar by Nelson-Somogyi Method
Working Principle: The reducing sugars when heated with alkaline copper tartrate reduce the copper from the cupric to cuprous state and thus cuprous oxide is formed. When cuprous oxide is treated with arsenomolybdic acid, the reduction of molybdic acid to molybdenum blue takes place. The blue colour developed is compared with a set of standards in a spectrophotometer at 620 nm
Application/ Scope: The Nelson-Somogyi method is one of the classical and widely used methods for the quantitative determination of reducing sugars like glucose, galactose, lactose and maltose in samples.Total reducing sugar in the sample will be expressed in mg/g or percentage
Sample Requirement: 5gm of brown/white rice
Charges: Rs.1320 per sample