Name of Parameter: Total Soluble Sugar by Anthrone Method
Working Principle: The reducing sugars when heated with alkaline copper tartrate reduce the copper from the cupric to cuprous state and thus cuprous oxide is formed. When cuprous oxide is treated with arsenomolybdic acid, the reduction of molybdic acid to molybdenum blue takes place. The blue colour developed is compared with a set of standards in a spectrophotometer at 620 nm
Application/Scope: The carbohydrate content in the samples can be measured by hydrolysing the polysaccharides into simple sugars by acid hydrolysis and estimating the resultant monosaccharides. Total sugar content in the sample expressed in mg/g or percentage
Sample Requirement: 5gm of brown/white rice
Charges: Rs.1320 per sample